Exposure to sub-lethal 2,4-dichlorophenoxyacetic acid arrests cell division and alters cell surface properties in Escherichia coli

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dc.contributor.authorBhat, Supriya V.
dc.contributor.authorKamencic, Belma
dc.contributor.authorKörnig, André
dc.contributor.authorShahina, Zinnat
dc.contributor.authorDahms, Tanya E. S.
dc.date.accessioned2023-05-17T17:56:22Z
dc.date.available2023-05-17T17:56:22Z
dc.date.issued2018-02-01
dc.description© 2018 Bhat, Kamencic, Körnig, Shahina and Dahms. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractEscherichia coli is a robust, easily adaptable and culturable bacterium in vitro, and a model bacterium for studying the impact of xenobiotics in the environment. We have used correlative atomic force – laser scanning confocal microscopy (AFM-LSCM) to characterize the mechanisms of cellular response to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). One of the most extensively used herbicides world-wide, 2,4-D is known to cause hazardous effects in diverse non-target organisms. Sub-lethal concentrations of 2,4-D caused DNA damage in E. coli WM1074 during short exposure periods which increased significantly over time. In response to 2,4-D, FtsZ and FtsA relocalized within seconds, coinciding with the complete inhibition of cell septation and cell elongation. Exposure to 2,4-D also resulted in increased activation of the SOS response. Changes to cell division were accompanied by concomitant changes to surface roughness, elasticity and adhesion in a time-dependent manner. This is the first study describing the mechanistic details of 2,4-D at sub-lethal levels in bacteria. Our study suggests that 2,4-D arrests E. coli cell division within seconds after exposure by disrupting the divisome complex, facilitated by dissipation of membrane potential. Over longer exposures, 2,4-D causes filamentation as a result of an SOS response to oxidative stress induced DNA damage.en_US
dc.description.authorstatusFacultyen_US
dc.description.peerreviewyesen_US
dc.description.sponsorshipThis work was supported by a Natural Sciences and Engineering Research Council (NSERC) Discovery Grant (228206-07) and Canada Foundation for Innovation Award to TD. SB and ZS were partially supported by scholarships from the Faculty of Graduate Studies and Research and the Department of Chemistry and Biochemistry (U. Regina), and BK was supported by NSERC Undergraduate Student Research Assistantship.en_US
dc.identifier.citationBhat SV, Kamencic B, Körnig A, Shahina Z and Dahms TES (2018) Exposure to Sub-lethal 2,4-Dichlorophenoxyacetic Acid Arrests Cell Division and Alters Cell Surface Properties in Escherichia coli. Front. Microbiol. 9:44. doi: 10.3389/fmicb.2018.00044en_US
dc.identifier.doihttps://doi.org/10.3389/fmicb.2018.00044
dc.identifier.urihttps://hdl.handle.net/10294/15927
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject2,4-dichlorophenoxyacetic aciden_US
dc.subjectcell divisionen_US
dc.subjectcorrelated atomic force – laser scanning confocal microscopy (AFM-LSCM)en_US
dc.subjectDNA damageen_US
dc.subjectFtsAen_US
dc.subjectFtsZen_US
dc.subjectmembrane potentialen_US
dc.subjectSOS responseen_US
dc.titleExposure to sub-lethal 2,4-dichlorophenoxyacetic acid arrests cell division and alters cell surface properties in Escherichia colien_US
dc.typeArticleen_US
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