Proteomic Analysis of Salmonella-modified Membranes Reveals Adaptations to Macrophage Hosts*

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dc.contributor.authorReuter, Tatjana
dc.contributor.authorVorwerk, Stephanie
dc.contributor.authorLiss, Viktoria
dc.contributor.authorChao, Tzu-Chiao
dc.contributor.authorHensel, Michael
dc.contributor.authorHansmeier, Nicole
dc.date.accessioned2023-05-23T19:28:54Z
dc.date.available2023-05-23T19:28:54Z
dc.date.issued2020-05
dc.description© 2020 Reuter et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. You are not required to obtain permission to reuse this article.en_US
dc.description.abstractSystemic infection and proliferation of intracellular pathogens require the biogenesis of a growth-stimulating compartment. The gastrointestinal pathogen Salmonella enterica commonly forms highly dynamic and extensive tubular membrane compartments built from Salmonella-modified membranes (SMMs) in diverse host cells. Although the general mechanism involved in the formation of replication-permissive compartments of S. enterica is well researched, much less is known regarding specific adaptations to different host cell types. Using an affinity-based proteome approach, we explored the composition of SMMs in murine macrophages. The systematic characterization provides a broader landscape of host players to the maturation of Salmonella-containing compartments and reveals core host elements targeted by Salmonella in macrophages as well as epithelial cells. However, we also identified subtle host specific adaptations. Some of these observations, such as the differential involvement of the COPII system, Rab GTPases 2A, 8B, 11 and ER transport proteins Sec61 and Sec22B may explain cell line-dependent variations in the pathophysiology of Salmonella infections. In summary, our system-wide approach demonstrates a hitherto underappreciated impact of the host cell type in the formation of intracellular compartments by Salmonella.en_US
dc.description.authorstatusFacultyen_US
dc.description.peerreviewyesen_US
dc.identifier.citationReuter T., Vorwerk S., Liss V., Chao T.C., Hensel M., Hansmeier N*. (2020) Proteomic analysis of Salmonella-modified membranes reveals adaptations to macrophage hosts. Molecular and Cellular Proteomics 19(5):900-912. https://doi.org/10.1074/mcp.RA119.001841en_US
dc.identifier.doihttps://doi.org/10.1074/mcp.RA119.001841
dc.identifier.urihttps://hdl.handle.net/10294/15933
dc.language.isoenen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology (ASBMB)en_US
dc.rightsAttribution 4.0 United States*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleProteomic Analysis of Salmonella-modified Membranes Reveals Adaptations to Macrophage Hosts*en_US
dc.typeArticleen_US
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