Metabolic adaptation of C-terminal protease A-deficient R. leguminosarum in response to loss of nutrient transport

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dc.contributor.authorJun, Dong
dc.contributor.authorMinic, Zoran
dc.contributor.authorBhat, Supriya V.
dc.contributor.authorVanderlinde, Elizabeth M.
dc.contributor.authorYost, Chris K.
dc.contributor.authorBabu, Mohan
dc.contributor.authorDahms, Tanya E. S.
dc.date.accessioned2023-05-17T19:22:43Z
dc.date.available2023-05-17T19:22:43Z
dc.date.issued2018-01-04
dc.description© 2018 Jun, Minic, Bhat, Vanderlinde, Yost, Babu and Dahms. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractPost-translational modification expands the functionality of the proteome beyond genetic encoding, impacting many cellular processes. Cleavage of the carboxyl terminus is one of the many different ways proteins can be modified for functionality. Gel-electrophoresis and mass spectrometric-based techniques were used to identify proteins impacted by deficiency of a C-terminal protease, CtpA, in Rhizobium leguminosarum bv. viciae 3841. Predicted CtpA substrates from 2D silver stained gels were predominantly outer membrane and transport proteins. Proteins with altered abundance in the wild type and ctpA (RL4692) mutant, separated by 2D difference gel electrophoresis, were selected for analysis by mass spectrometry. Of those identified, 9 were the periplasmic solute-binding components of ABC transporters, 5 were amino acid metabolic enzymes, 2 were proteins involved in sulfur metabolism, and 1 each was related to carbon metabolism, protein folding and signal transduction. Alterations to ABC-binding-cassette transporters, nutrient uptake efficiency and to amino acid metabolism indicated an impact on amino acid metabolism and transport for the ctpA mutant, which was validated by measured amino acid levels.en_US
dc.description.authorstatusFacultyen_US
dc.description.peerreviewyesen_US
dc.description.sponsorshipThis work was supported by the Natural Sciences and Engineering Research Discovery Grants to TD (228206-2011), CY (288281-2017) and MB (20234-2012), and a Canada Foundation for Innovation Leaders Opportunity Fund to TD and CY.en_US
dc.identifier.citationJun D, Minic Z, Bhat SV, Vanderlinde EM, Yost CK, Babu M and Dahms TES (2018) Metabolic Adaptation of a C-Terminal Protease A-Deficient Rhizobium leguminosarum in Response to Loss of Nutrient Transport. Front. Microbiol. 8:2617. doi: 10.3389/fmicb.2017.02617en_US
dc.identifier.doihttps://doi.org/10.3389/fmicb.2017.02617
dc.identifier.urihttps://hdl.handle.net/10294/15928
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectABC transportersen_US
dc.subjectamino acid metabolismen_US
dc.subjectC-terminal proteaseen_US
dc.subjectproteomicsen_US
dc.subjectRhizobium leguminosarumen_US
dc.titleMetabolic adaptation of C-terminal protease A-deficient R. leguminosarum in response to loss of nutrient transporten_US
dc.typeArticleen_US
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