EF1025, a hypothetical protein from Enterococcus faecalis, interacts with DivIVA and affects cell length and cell shape

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dc.contributor.authorSharma, Kusum
dc.contributor.authorSultana, Taranum
dc.contributor.authorLiao, Mingmin
dc.contributor.authorDahms, Tanya E. S.
dc.contributor.authorDillon, Jo-Anne R.
dc.date.accessioned2023-05-15T19:35:39Z
dc.date.available2023-05-15T19:35:39Z
dc.date.issued2020-02-12
dc.description© 2020 Sharma, Sultana, Liao, Dahms and Dillon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractDivIVA plays multifaceted roles in Gram-positive organisms through its association with various cell division and non-cell division proteins. We report a novel DivIVA interacting protein in Enterococcus faecalis, named EF1025 (encoded by EF1025), which is conserved in Gram-positive bacteria. The interaction of EF1025 with DivIVAEf was confirmed by Bacterial Two-Hybrid, Glutathione S-Transferase pull-down, and co-immunoprecipitation assays. EF1025, which contains a DNA binding domain and two Cystathionine β-Synthase (CBS) domains, forms a decamer mediated by the two CBS domains. Viable cells were recovered after insertional inactivation or deletion of EF1025 only through complementation of EF1025 in trans. These cells were longer than the average length of E. faecalis cells and had distorted shapes. Overexpression of EF1025 also resulted in cell elongation. Immuno-staining revealed comparable localization patterns of EF1025 and DivIVAEf in the later stages of division in E. faecalis cells. In summary, EF1025 is a novel DivIVA interacting protein influencing cell length and morphology in E. faecalis.en_US
dc.description.authorstatusFacultyen_US
dc.description.peerreviewyesen_US
dc.description.sponsorshipThis work was supported by the Natural Science and Engineering Research Council of Canada (NSERC, Grant RGPIN-203651-2006 to J-AD and RGPIN-2018-06649 to TD) and the Sick Kids Foundation (Grant XG02-075R to J-AD).en_US
dc.identifier.citationSharma K., Sultana T., Liao M., Dahms T. E. S., Dillon J.-A. R.* (2020) EF1025, a hypothetical protein from Enterococcus faecalis, interacts with DivIVA and affects cell length and cell shape. Frontiers in Microbiology 11:83. https://doi.org/10.3389/fmicb.2020.00083en_US
dc.identifier.doihttps://doi.org/10.3389/fmicb.2020.00083
dc.identifier.urihttps://hdl.handle.net/10294/15921
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectGram-positive bacteriaen_US
dc.subjectEnterococcus faecalisen_US
dc.subjectcell divisionen_US
dc.subjectDivIVAen_US
dc.subjectprotein–protein interactionen_US
dc.subjectBacillus subtilisen_US
dc.titleEF1025, a hypothetical protein from Enterococcus faecalis, interacts with DivIVA and affects cell length and cell shapeen_US
dc.typeArticleen_US
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