Purification and proteomics of pathogen-modified vacuoles and membranes

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dc.contributor.authorHerweg, Jo-Ana
dc.contributor.authorHansmeier, Nicole
dc.contributor.authorOtto, Andreas
dc.contributor.authorGeffken, Anna C
dc.contributor.authorSubbarayal, Prema
dc.contributor.authorPrusty, Bhupesh K
dc.contributor.authorBecher, Dörte
dc.contributor.authorHensel, Michael
dc.contributor.authorSchaible, Ulrich E
dc.contributor.authorRudel, Thomas
dc.contributor.authorHilbi, Hubert
dc.date.accessioned2023-05-30T15:16:57Z
dc.date.available2023-05-30T15:16:57Z
dc.date.issued2015-06-02
dc.description© 2015 Herweg, Hansmeier, Otto, Geffken, Subbarayal, Prusty, Becher, Hensel, Schaible, Rudel and Hilbi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractCertain pathogenic bacteria adopt an intracellular lifestyle and proliferate in eukaryotic host cells. The intracellular niche protects the bacteria from cellular and humoral components of the mammalian immune system, and at the same time, allows the bacteria to gain access to otherwise restricted nutrient sources. Yet, intracellular protection and access to nutrients comes with a price, i.e., the bacteria need to overcome cell-autonomous defense mechanisms, such as the bactericidal endocytic pathway. While a few bacteria rupture the early phagosome and escape into the host cytoplasm, most intracellular pathogens form a distinct, degradation-resistant and replication-permissive membranous compartment. Intracellular bacteria that form unique pathogen vacuoles include Legionella, Mycobacterium, Chlamydia, Simkania, and Salmonella species. In order to understand the formation of these pathogen niches on a global scale and in a comprehensive and quantitative manner, an inventory of compartment-associated host factors is required. To this end, the intact pathogen compartments need to be isolated, purified and biochemically characterized. Here, we review recent progress on the isolation and purification of pathogen-modified vacuoles and membranes, as well as their proteomic characterization by mass spectrometry and different validation approaches. These studies provide the basis for further investigations on the specific mechanisms of pathogen-driven compartment formation.en_US
dc.description.authorstatusFacultyen_US
dc.description.peerreviewyesen_US
dc.description.sponsorshipThis collaborative effort was funded by the “Bundesministerium für Bildung und Forschung” (BMBF) “Medical Infection Genomics” initiative (0315834C-D) project “Pathogen-Host Interactomes.” We gratefully acknowledge further funding for the group of H.H. from the German Research Foundation (DFG; HI 1511/1-1, SPP1580), and the Swiss National Science Foundation (SNF; 31003A_153200). The work of the T.R. team was funded by the DFG (Simkania; SPP1580 RU631/9-1, RU631/9-2) and the BMBF (Chlamydia; “Medical Infection Genomics” initiative, 0315834A). The group of U.E.S. was funded by the DFG (Scha 514/2–1 and SPP1580 Scha 514/3-1), and the teams of M.H. and N.H. by the DFG through grant HE1964/18-1 as part of SPP1580. A.O. and D.B. were funded by the BMBF (ZIK 03Z1CN21).en_US
dc.identifier.citationHerwig J.-A., Hansmeier N., Otto A., Geffken A., Subbarayal P., Prusty B.K., Becher D., Hensel M., Schaible U.E., Rudel T., Hilbi H. (2015). Purification and proteomics of pathogen-modified vacuoles and membranes. Frontiers in Cellular and Infection Microbiology 5, 48. https://doi.org/10.3389/fcimb.2015.00048en_US
dc.identifier.doihttps://doi.org/10.3389/fcimb.2015.00048
dc.identifier.urihttps://hdl.handle.net/10294/15943
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectChlamydiaen_US
dc.subjecthost-pathogen interactionsen_US
dc.subjectimmuno-magnetic purificationen_US
dc.subjectLegionellaen_US
dc.subjectMycobacteriumen_US
dc.subjectSalmonellaen_US
dc.subjectSimkaniaen_US
dc.subjectpathogen vacuoleen_US
dc.titlePurification and proteomics of pathogen-modified vacuoles and membranesen_US
dc.typeArticleen_US
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