AFM-based correlative microscopy illuminates human pathogens

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dc.contributor.authorBhat, Supriya V.
dc.contributor.authorPrice, Jared D. W.
dc.contributor.authorDahms, Tanya E. S.
dc.date.accessioned2023-05-09T17:41:49Z
dc.date.available2023-05-09T17:41:49Z
dc.date.issued2021-05-07
dc.description© 2021 Bhat, Price and Dahms. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractMicrobes have an arsenal of virulence factors that contribute to their pathogenicity. A number of challenges remain to fully understand disease transmission, fitness landscape, antimicrobial resistance and host heterogeneity. A variety of tools have been used to address diverse aspects of pathogenicity, from molecular host-pathogen interactions to the mechanisms of disease acquisition and transmission. Current gaps in our knowledge include a more direct understanding of host-pathogen interactions, including signaling at interfaces, and direct phenotypic confirmation of pathogenicity. Correlative microscopy has been gaining traction to address the many challenges currently faced in biomedicine, in particular the combination of optical and atomic force microscopy (AFM). AFM, generates high-resolution surface topographical images, and quantifies mechanical properties at the pN scale under physiologically relevant conditions. When combined with optical microscopy, AFM probes pathogen surfaces and their physical and molecular interaction with host cells, while the various modes of optical microscopy view internal cellular responses of the pathogen and host. Here we review the most recent advances in our understanding of pathogens, recent applications of AFM to the field, how correlative AFM-optical microspectroscopy and microscopy have been used to illuminate pathogenicity and how these methods can reach their full potential for studying host- pathogen interactions.en_US
dc.description.authorstatusFacultyen_US
dc.description.peerreviewyesen_US
dc.description.sponsorshipSB was funded by a Natural Science and Engineering Research Council of Canada Discovery grant to TD (RGPIN-2018-06649).en_US
dc.identifier.citationBhat S. V., Price J. D. W., Dahms T. E. S.* (2021) AFM-based correlative microscopy illuminates human pathogens. Frontiers in Cellular and Infection Microbiology 11, 655501. doi: 10.3389/fcimb.2021.655501en_US
dc.identifier.doihttps://doi.org/10.3389/fcimb.2021.655501
dc.identifier.urihttps://hdl.handle.net/10294/15914
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectatomic force microscopy (AFM)en_US
dc.subjectbacteriaen_US
dc.subjectcorrelative microspectroscopy and microscopyen_US
dc.subjectfungien_US
dc.subjecthost- pathogen interactionen_US
dc.subjectmicrobesen_US
dc.subjectpathogenicityen_US
dc.subjectvirusesen_US
dc.titleAFM-based correlative microscopy illuminates human pathogensen_US
dc.typeArticleen_US
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