Rhizobium leguminosarum bv. viciae 3841 Adapts to 2,4-Dichlorophenoxyacetic Acid with “Auxin-Like” Morphological Changes, Cell Envelope Remodeling and Upregulation of Central Metabolic Pathways
| dc.contributor.author | Bhat, Supriya V. | |
| dc.contributor.author | Booth, Sean C. | |
| dc.contributor.author | McGrath, Seamus G. K. | |
| dc.contributor.author | Dahms, Tanya E. S. | |
| dc.date.accessioned | 2023-05-16T21:24:13Z | |
| dc.date.available | 2023-05-16T21:24:13Z | |
| dc.date.issued | 2015-04-28 | |
| dc.description | © 2015 Bhat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
| dc.description.abstract | There is a growing need to characterize the effects of environmental stressors at the molec- ular level on model organisms with the ever increasing number and variety of anthropogenic chemical pollutants. The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), as one of the most widely applied pesticides in the world, is one such example. This herbicide is known to have non-targeted undesirable effects on humans, animals and soil microbes, but specific molecular targets at sublethal levels are unknown. In this study, we have used Rhizobium leguminosarum bv. viciae 3841 (Rlv) as a nitrogen fixing, beneficial model soil organism to characterize the effects of 2,4-D. Using metabolomics and advanced microscopy we deter- mined specific target pathways in the Rlv metabolic network and consequent changes to its phenotype, surface ultrastructure, and physical properties during sublethal 2,4-D exposure. Auxin and 2,4-D, its structural analogue, showed common morphological changes in vitro- which were similar to bacteroids isolated from plant nodules, implying that these changes are related to bacteroid differentiation required for nitrogen fixation. Rlv showed remarkable adaptation capabilities in response to the herbicide, with changes to integral pathways of cellular metabolism and the potential to assimilate 2,4-D with consequent changes to its physical and structural properties. This study identifies biomarkers of 2,4-D in Rlv and offers valuable insights into the mode-of-action of 2,4-D in soil bacteria. | en_US |
| dc.description.authorstatus | Faculty | en_US |
| dc.description.peerreview | yes | en_US |
| dc.description.sponsorship | This work was supported by the National Science and Engineering Research Council of Canada Discovery Grant (228206-2012) to TESD, and the Canada Foundation for Innovation Leaders Opportunity Fund (29962) to TESD. | en_US |
| dc.identifier.citation | Bhat SV, Booth SC, McGrath SGK, Dahms TES (2015) Rhizobium leguminosarum bv. viciae 3841 Adapts to 2,4-Dichlorophenoxyacetic Acid with “Auxin-Like” Morphological Changes, Cell Envelope Remodeling and Upregulation of Central Metabolic Pathways. PLoS ONE 10(4): e0123813. doi:10.1371/ journal.pone.0123813 | en_US |
| dc.identifier.doi | https://doi.org/10.1371/journal.pone.0123813 | |
| dc.identifier.uri | https://hdl.handle.net/10294/15924 | |
| dc.language.iso | en | en_US |
| dc.publisher | Public Library of Science | en_US |
| dc.rights | Attribution 4.0 United States | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.title | Rhizobium leguminosarum bv. viciae 3841 Adapts to 2,4-Dichlorophenoxyacetic Acid with “Auxin-Like” Morphological Changes, Cell Envelope Remodeling and Upregulation of Central Metabolic Pathways | en_US |
| dc.type | Article | en_US |