Proteomics of intracellular Salmonella enterica reveals roles of Salmonella pathogenicity island 2 in metabolism and antioxidant defense

Simple item page
dc.contributor.authorNoster, Janina
dc.contributor.authorChao, Tzu-Chiao
dc.contributor.authorSander, Nathalie
dc.contributor.authorSchulte, Marc
dc.contributor.authorReuter, Tatjana
dc.contributor.authorHansmeier, Nicole
dc.contributor.authorHensel, Michael
dc.date.accessioned2023-05-23T19:01:44Z
dc.date.available2023-05-23T19:01:44Z
dc.date.issued2019-04-22
dc.description© 2019 Noster et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.abstractIntracellular Salmonella enterica serovar Typhimurium (STM) deploy the Salmonella Pathogenicity Island 2-encoded type III secretion system (SPI2-T3SS) for the massive remodeling of the endosomal system for host cells. This activity results in formation of an extensive interconnected tubular network of Salmonella-induced filaments (SIFs) connected to the Salmonella-containing vacuole (SCV). Such network is absent in cells infected with SPI2-T3SS-deficient mutant strains such as ΔssaV. A tubular network with reduced dimensions is formed if SPI2-T3SS effector protein SseF is absent. Previous single cell live microscopy-based analyses revealed that intracellular proliferation of STM is directly correlated to the ability to transform the host cell endosomal system into a complex tubular network. This network may also abrogate host defense mechanisms such as delivery of antimicrobial effectors to the SCV. To test the role of SIFs in STM patho-metabolism, we performed quantitative comparative proteomics of STM recovered from infected murine macrophages. We infected RAW264.7 cells with STM wild type (WT), ΔsseF or ΔssaV strains, recovered bacteria 12 h after infection and determined proteome compositions. Increased numbers of proteins characteristic for nutritional starvation were detected in STM ΔsseF and ΔssaV compared to WT. In addition, STM ΔssaV, but not ΔsseF showed signatures of increased exposure to stress by antimicrobial defenses, in particular reactive oxygen species, of the host cells. The proteomics analyses presented here support and extend the role of SIFs for the intracellular lifestyle of STM. We conclude that efficient manipulation of the host cell endosomal system by effector proteins of the SPI2-T3SS contributes to nutrition, as well as to resistance against antimicrobial host defense mechanisms.en_US
dc.description.authorstatusFacultyen_US
dc.description.peerreviewyesen_US
dc.description.sponsorshipThis work was supported by grant P15 of SFB 944 of the Deutsche Forschungsgemeinschaft (http://www.dfg.de/) and Infect-ERA project ‘SalHostTrop’ (http://www.infect-era.eu/) to MH. We gratefully acknowledge the support by the MWK of Lower Saxony by an installation grant. NH was supported in part by intramural funding of the school of Biology/Chemistry of the University of Osnabrück (https://www.biologie.uni-osnabrueck.de/biologie.html). Ten_US
dc.identifier.citationNoster J, Chao T-C, Sander N, Schulte M, Reuter T, Hansmeier N, et al. (2019) Proteomics of intracellular Salmonella enterica reveals roles of Salmonella pathogenicity island 2 in metabolism and antioxidant defense. PLoS Pathog 15(4): e1007741. https://doi.org/10.1371/journal. ppat.1007741en_US
dc.identifier.doihttps://doi.org/10.1371/journal. ppat.1007741
dc.identifier.urihttps://hdl.handle.net/10294/15930
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleProteomics of intracellular Salmonella enterica reveals roles of Salmonella pathogenicity island 2 in metabolism and antioxidant defenseen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Proteomics of intracellular Salmonella enterica.pdf
Size:
3.48 MB
Format:
Adobe Portable Document Format
Description:
Published version (PDF)
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Nicole Hansmeier