Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium
Date
2021-03-09
Authors
Barrila, Jennifer
Sarker, Shameema F.
Hansmeier, Nicole
Yang, Shanshan
Buss, Kristina
Briones, Natalia
Park, Jin
Davis, Richard R.
Forsyth, Rebecca J.
Ott, C. Mark
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Research
Abstract
Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE study flown aboard Space Shuttle mission STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection with Salmonella Typhimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this was the first in-flight infection and dual RNA-seq analysis using human cells.
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Citation
Barrila, J., Sarker, S.F., Hansmeier, N. et al. Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium. npj Microgravity 7, 9 (2021). https://doi.org/10.1038/s41526-021-00136-w